Lab Pichia Pastoris Expressed Recombinant Human Apo Transferrin Iron Deprivation Research

ART201S Recombinant Human Apo-Transferrin

Animal-Free, Pichia pastoris Expressed | Precision Iron Regulation for Cell Culture

Precise iron homeostasis is critical for serum-free media formulation, cell metabolism research and biopharmaceutical process development. Traditional plasma-derived apo-transferrin suffers from inherent blood-borne contamination risks and high batch-to-batch variability, while small-molecule chelators such as EDTA cause non-specific cytotoxicity and oxidative stress, limiting their use in sensitive culture systems.

Anrate ART201S recombinant human apo-transferrin is a high-purity, iron-free iron regulatory protein expressed in a Pichia pastoris system. With ultra-low basal iron residue and intact iron-binding activity, it enables accurate control of extracellular iron concentration without cellular toxicity. It serves as a physiologically compatible, regulatory-friendly alternative to plasma-derived apo-transferrin and chemical chelators in serum-free culture, iron metabolism research and biomanufacturing workflows.

Key Advantages

 I.High Iron-Binding Activity, Ultra-Low Iron Residue

Each molecule reliably binds two ferric ions with full biological activity. Basal iron residue is controlled below 0.01%, enabling precise gradient regulation of iron concentration and highly reproducible iron deprivation and cell synchronization experiments.

II.100% Animal-Free, Zero Blood-Borne Risk

Produced via recombinant Pichia pastoris expression with no animal-derived raw materials throughout manufacturing. Eliminates all blood-borne pathogen risks and fully complies with FDA, EMA and ICH guidelines for biopharmaceutical raw materials.

III.High Purity & Ultra-Low Endotoxin

Purified through multi-step chromatography with ≥98% homogeneity by HPLC. Endotoxin level is ≤ 0.5 EU/mg, with no mycoplasma or microbial contamination, minimizing inflammatory response and supporting stem cell, immune cell and other sensitive culture systems.

IV.Superior Batch-to-Batch Consistency

Standardized fermentation and purification processes ensure consistent iron-binding capacity, protein concentration and bioactivity across all lots. It eliminates the variability inherent to plasma-derived products and guarantees stable, repeatable process performance.

V.Physiologically Friendly, No Oxidative Cytotoxicity

Unlike non-specific chemical chelators, apo-transferrin regulates iron levels via the physiological transferrin receptor pathway. It does not disrupt cellular redox balance or induce reactive oxygen species, making it highly compatible with long-term high-density cell culture.

VI.Scalable Specifications, Seamless Scale-Up

Available in 1 g, 10 g and 100 g pack sizes, with custom specifications available on request. Large-scale in-house production capacity supports seamless transition from lab research to industrial biomanufacturing.

Technical Specifications

Why Choose Anrate?

Anrate specializes in recombinant protein R&D and manufacturing with a proprietary Pichia pastoris expression platform. We control the entire production chain from gene construction to final fill-finish, ensuring stable supply, competitive pricing and full traceability. Every batch is released with a comprehensive Certificate of Analysis (CoA), and our technical team provides application support for media optimization and process scale-up. We serve customers across North America, Europe and Asia-Pacific with reliable cold-chain logistics and responsive technical service.

Frequently Asked Questions

Q: What is the difference between apo-transferrin and holo-transferrin? How do I choose?

A: Holo-transferrin is fully iron-saturated and serves as a direct iron source for cells. Apo-transferrin is iron-free and is used to chelate free iron and precisely regulate iron concentration in culture systems. Choose holo-transferrin when you need to supplement iron, and apo-transferrin when you need to reduce or control iron levels.

Q: How do I load iron onto recombinant apo-transferrin?

A: Apo-transferrin can be saturated with ferric ions under controlled pH and buffer conditions. Our technical team provides detailed iron-loading protocols upon request.

Q: Is this product suitable for GMP bioproduction?

A: ART201S is manufactured under a strict quality management system and is widely used in pre-clinical and clinical-stage bioprocess development. Please contact our team for GMP-grade product options.

Q: What is the shelf life after reconstitution?

A: Reconstituted sterile solution can be stored at 2–8 °C for up to 30 days. Aliquot to avoid repeated freeze-thaw cycles and contamination.

Q: Do you offer custom packaging and bulk pricing?

A: Yes, we support custom specifications and volume-based pricing for industrial-scale orders. Please contact our sales team for a detailed quotation.

Disclaimer

This product is intended exclusively for scientific research experiments, laboratory investigation and industrial production purposes. It is strictly prohibited for use in clinical therapeutic applications, and must not be administered to animals or human beings.

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WhatsApp: +85363312841 | Email: sales@bjanrate.cn