Biomanufacturing Recombinant Holo Transferrin Fully Iron Saturated

ART202S Recombinant Holo-Transferrin

Animal-Free, Pichia pastoris Expressed | Ready-to-Use Iron Source for Mammalian Cell Culture

Efficient, physiologically relevant iron delivery is a cornerstone of serum-free cell culture, directly impacting cell proliferation, viability and recombinant protein yield. Traditional plasma-derived holo-transferrin carries inherent blood-borne contamination risks and batch-to-batch variability, while inorganic iron salts cause non-specific oxidative stress and low bioavailability, limiting cell culture performance and process scalability.

Anrate ART202S recombinant holo-transferrin is a fully iron-saturated iron transport protein expressed in a Pichia pastoris system. Pre-loaded with two ferric ions per molecule, it delivers bioavailable iron directly to cells via the transferrin receptor pathway. As a 100% animal-free, ready-to-use iron source, it serves as a high-performance, regulatory friendly replacement for plasma-derived transferrin and inorganic iron salts in serum-free media and industrial biomanufacturing.

Key Advantages

I.Fully Iron-Saturated Holo Form, Ready to Use

Each transferrin molecule is pre-loaded with two ferric ions at full saturation. No additional iron chelation or loading is required — simply reconstitute and add to media, streamlining formulation workflows and reducing process variables.

II.100% Animal-Free, Zero Blood-Borne Risk

Produced via recombinant Pichia pastoris expression with zero animal-derived raw materials throughout the manufacturing process. Eliminates all blood-borne pathogen risks and fully complies with FDA, EMA and ICH guidelines for biopharmaceutical raw materials.

III.High Purity & Ultra-Low Endotoxin

Purified through multi-step chromatography with ≥98% homogeneity by HPLC. Endotoxin level is controlled below 0.5 EU/mg, minimizing inflammatory response and cytotoxicity, and ensuring compatibility with sensitive cell lines, stem cells and immune cell culture.

IV.Superior Batch-to-Batch Consistency

Standardized fermentation and purification processes guarantee consistent iron saturation, protein concentration and biological activity across every production lot. Reliable batch consistency delivers reproducible cell growth and stable protein expression run after run.

V.Physiological Iron Delivery, No Oxidative Stress

Iron is delivered exclusively via the transferrin receptor (TfR) mediated endocytosis pathway, matching natural cellular iron metabolism. Unlike inorganic iron salts, it avoids non-specific uptake and oxidative damage, supporting healthier, higher-density cell culture.

VI.Scalable Packaging, Seamless Scale-Up

Available in 1 g, 10 g and 100 g pack sizes, with custom specifications available on request. Large-scale in-house production capacity supports seamless transition from lab-scale research to industrial biomanufacturing.

Technical Specifications

Typical Applications

• Serum-Free & Chemically Defined Media Formulation
  Serves as the primary physiological iron source to replace plasma-derived transferrin, enabling fully animal-free, chemically defined culture systems for antibody , vaccine and cell therapy media development.
• Biopharmaceutical High-Density Bioproduction
  Supports high-density suspension culture of CHO, HEK293 and other engineering cell lines, enhancing cell viability, proliferation rate and recombinant protein / antibody expression yield.
• Stem Cell & Immune Cell Culture
  Low-endotoxin, xeno-free formulation supports the expansion of mesenchymal stem cells, iPSCs, CAR-T and NK cells, meeting the high safety and consistency requirements of cell therapy development.
• In Vitro Diagnostic (IVD) Raw Material
  Used as a key component in cell culture-based diagnostic kits and biochemical assay systems, with consistent batch quality to ensure stable assay performance.
• Basic Cell Biology Research
  Applied in cell metabolism, proliferation, iron homeostasis and signal transduction research, providing a defined, contaminant-free iron source for reproducible experimental results.

Why Choose Anrate?

Anrate specializes in recombinant protein R&D and manufacturing with a proprietary Pichia pastoris expression platform. We control the entire production chain from gene construction to final fill-finish, ensuring stable supply, competitive pricing and full traceability. Every batch is released with a comprehensive Certificate of Analysis (CoA), and our technical team provides application support for media optimization and process scale-up. We serve customers across North America, Europe and Asia-Pacific with reliable cold-chain logistics and responsive technical service.

Frequently Asked Questions

Q: What is the difference between holo-transferrin and apo-transferrin? How do I choose?

A: Holo-transferrin is fully iron-saturated and is used as a direct iron source to supplement cell culture media. Apo-transferrin is iron-free and is used to chelate free iron and regulate iron concentration. Choose holo-transferrin when you need to add iron, and apo-transferrin when you need to reduce or control iron levels.

Q: Can this product be added directly to cell culture media?

A: Yes. ART202S is supplied in a fully iron-saturated holo form. After reconstitution with sterile PBS or suitable buffer, it can be added directly to culture media at the desired working concentration.

Q: Is this product suitable for GMP bioproduction?

A: ART202S is manufactured under a strict quality management system and is widely used in pre-clinical and clinical-stage bioprocess development. Please contact our team for GMP-grade product options.

Q: What is the shelf life after reconstitution?

A: Reconstituted sterile solution can be stored at 2–8 °C for up to 30 days. Aliquot to avoid repeated freeze-thaw cycles and contamination.

Q: Do you offer custom packaging and bulk pricing?

A: Yes, we support custom specifications and volume-based pricing for industrial-scale orders. Please contact our sales team for a detailed quotation.