Recombinant Human Holo-Transferrin CAS 11096-37-0 | Serum-Free Grade

Product Overview

Anrate Recombinant Human Holo-Transferrin (CAS 11096-37-0) is a high-purity, iron-saturated recombinant protein formulated specifically for serum-free cell culture and in vitro bioresearch workflows. Produced via advanced recombinant expression technology and multi-step chromatographic purification, this serum-free grade holo-transferrin delivers consistent iron-binding activity and exceptional batch-to-batch reproducibility, serving as a chemically defined alternative to plasma-derived transferrin products.

As a fully iron-loaded (holo) form of human serotransferrin, this product mediates efficient cellular iron uptake through transferrin receptor pathways, supporting stable cell proliferation and metabolic function. It eliminates the contamination risks and performance variability associated with animal serum-derived raw materials, making it ideal for sensitive mammalian cell culture, cell line development, and bioprocess optimization applications.

Key Features & Advantages

• High-Purity Recombinant Expression: Manufactured through a well-characterized expression system with ≥98% purity verified by SDS-PAGE and HPLC, free of animal-origin components and blood-borne pathogen risks.
• Fully Iron-Loaded Holo Form: Standard iron content of 0.16% ensures stable and consistent iron delivery capacity. It eliminates the need for additional iron supplementation in most culture formulations and reduces free iron-induced oxidative stress.
• Serum-Free Grade Formulation: Optimized for chemically defined, xeno-free and serum-free media systems, providing a traceable, reproducible supplement to simplify media development and scale-up workflows.
• Ultra-Low Endotoxin: Rigorously tested to contain ≤ 0.5 EU/mg endotoxin, compatible with sensitive cell lines and long-term continuous culture.
• Guaranteed Batch Consistency: Standardized production and full QC protocols minimize lot-to-lot variation, supporting reliable, repeatable research and process development results.

Applications

• Core supplementation for serum-free, chemically defined and protein-free cell culture media
• Routine maintenance and scale-up of mammalian cell lines (CHO, HEK293, hybridoma and stem cells)
• Cell line development and upstream bioprocess optimization for recombinant protein production
• In vitro studies of iron transport, cellular metabolism and protein-receptor interactions
• Formulation development for biopharmaceutical research and biomanufacturing process design

Product Specifications

Quality Assurance from Anrate