Mammalian Recombinant Apo Human Transferrin Protein High Purity Cell Culture Biomanufacturing

Recombinant Apo-Transferrin High-Purity Iron-Free Transferrin for Serum-Free Mammalian Cell Culture Biomanufacturing

Overview

Transferrin (TF) is an essential iron-binding protein that mediates intracellular iron delivery, regulates cellular respiration and proliferation, and provides inherent antioxidant and bactericidal protection in cell culture systems. Traditional plasma-derived transferrin carries inherent risks of animal-derived contaminants and high batch-to-batch variability, making it unsuitable for modern regulated bioproduction.

Anrate Recombinant Apo-Transferrin is expressed in Pichia pastoris via advanced recombinant DNA technology. As a fully animal-free, iron-free (apo-form) transferrin, it delivers exceptional consistency and safety, making it an ideal serum-free cell culture supplement for mammalian cell culture, biopharmaceutical manufacturing, cell & gene therapy, and vaccine production.

Key Product Advantages

I.100% Animal-Free, Eliminates Exogenous Contamination Risks

• No animal-derived components throughout the entire expression and purification process
• Eliminates the risk of blood-borne viruses, mycoplasma, and TSE/BSE contamination
• Fully compliant with regulatory requirements for biopharmaceutical manufacturing and advanced therapy products

II.Pichia pastoris Expressed, Superior Batch-to-Batch Consistency

• Standardized recombinant expression platform with fully traceable production workflow
• Highly stable purification process ensures minimal performance variation across lots
• Reduces process revalidation costs and supports long-term, reliable supply

III.High Purity & Low Endotoxin, Optimized for Sensitive Cell Lines

• HPLC purity ≥ 98.0% with negligible residual host cell protein
• Endotoxin level controlled below 0.5 EU/mg, minimizing cellular inflammatory response
• Clear, particle-free solution after reconstitution with no negative impact on cell growth

IV.Iron-Free Apo Form for Precise Iron Regulation

• Completely iron-free apo-transferrin with no bound iron ions
• Enables precise customization of iron concentration in culture media formulations
• Perfectly suited for inherently iron-rich basal media such as DMEM/F12, preventing iron overload and oxidative damage

Applications

This product is designed for serum-free and low-serum culture of various mammalian cell lines, supporting both academic research and industrial-scale bioproduction:

• Mammalian cell culture: CHO, HEK293, Vero, MDCK, BHK and other industrial cell lines
• Biopharmaceutical manufacturing: Monoclonal antibody, recombinant protein and biosimilar production
• Cell & Gene Therapy: Stem cell expansion, immune cell culture and advanced therapy process development
• Vaccine production: Viral vaccine process development and large-scale manufacturing
• Media formulation: Development and optimization of serum-free and chemically defined culture media

Quality Control Specifications

Handling & Storage

• Reconstitution: Dissolve the lyophilized powder in sterile PBS or other suitable solvent. A final concentration of ≤ 50 mg/mL is recommended. Filter through a sterile membrane filter before use.
• Storage: Store unopened product at 2–8°C in a tightly sealed, dry container, protected from light. Reconstituted sterile solution is stable for up to 30 days at 2–8°C.
• Precautions: Avoid repeated freeze-thaw cycles. Use promptly after opening to prevent microbial contamination.

Important Notice