ART202S Recombinant Holo Transferrin | Animal-Free Cell Culture Grade for Bioproduction

High-Purity Iron-Saturated Transferrin from Pichia pastoris | Reliable Bulk Supply

Product Overview

In serum-free cell culture systems, transferrin is a core functional component that mediates iron ion transport and maintains cell proliferation and metabolic homeostasis. Iron-saturated (holo) transferrin directly delivers bioavailable bound iron to cells without additional iron supplementation, making it particularly suitable for media formulations with low basal iron content and effectively streamlining formulation development. Traditional plasma-derived transferrin presents inherent limitations in source variability, biosafety risks and batch-to-batch inconsistency, making it difficult to meet the compliance and stability requirements of industrial-scale production.

Manufactured via recombinant Pichia pastoris eukaryotic expression and purification with a fully animal-free production process, ART202S recombinant human holo transferrin eliminates blood-source related risks at the source. Presented in iron-saturated holo form carrying two bound iron ions, it can be directly applied to various serum-free cell culture systems, supporting the development and large-scale manufacturing of chemically defined culture media.

Key Product Advantages

1. Animal-Free Process for Global Regulatory Compliance

The fully non-human, animal-free recombinant expression process ensures clear source traceability and full manufacturing control. It fully aligns with global regulatory expectations for chemically defined media raw materials. Complete quality documentation is available to support customers in process submission and CMC dossier preparation.

2. Iron-Saturated Holo Configuration for Streamlined Formulation Development

The iron-saturated holo form carries two bound iron ions, delivering directly bioavailable iron to cells without the need for additional iron salt supplementation in the media. It effectively simplifies formulation design, avoids oxidative stress and system instability caused by free iron ions, and facilitates faster process locking.

3. Comprehensive QC System for Excellent Batch Consistency

A strict release testing standard is in place, with every batch tested for multiple critical indicators including appearance, pH, purity, protein content, loss on drying and endotoxin. Controlled variation of key quality parameters across batches effectively reduces process validation costs and ensures stable, repeatable culture performance in large-scale production.

4. Multi-Specification Coverage for Full Lifecycle Scaling

Standard packaging options include 100mg, 1g, 10g and 100g, with customized packaging specifications available upon request. It covers dosage requirements across the entire lifecycle, from laboratory process development and pilot scale-up to commercial mass production.

Key Quality Specifications

Above data represents typical batch results. Actual values are subject to the Certificate of Analysis provided with delivered batches.

Industrial Advantages of Lyophilized Powder Formulation

• Extended Shelf Life: Up to 5-year validity for unopened products when stored at 2~8°C in a light-proof, airtight condition, supporting large-volume long-term stocking and reducing inventory turnover and procurement management costs
• Flexible Reconstitution: Recommended to be reconstituted with sterile PBS or suitable solvents up to 50mg/mL, followed by sterile filtration. It can be diluted and aliquoted on demand to meet different formulation concentration requirements
• Robust Logistics: The powder form eliminates liquid leakage risks, and quality stability can be maintained with conventional temperature-controlled transportation, greatly reducing long-distance logistics losses and delivery risks
• Convenient Usage: Reconstituted sterile solution can be stored at 2~8°C for 30 days, adapting to the rhythm of multi-batch continuous production

Target Applications

• Low-Iron / Iron-Free Basal Media Formulation: Delivers bound iron directly without additional iron salt supplementation, simplifies formulation composition, and supports the development of chemically defined serum-free media
• Serum-Free Cell Culture Systems: Serves as a core functional component to support in vitro proliferation and metabolic maintenance of various cell types including mammalian cells, stem cells and immune cells
• Process Development & Optimization: Well-defined composition with no animal-derived interference, suitable for formulation adjustment, process locking and comparability studies to shorten R&D and validation cycles
• Large-Scale Biopharmaceutical & CGT Manufacturing: Excellent batch-to-batch consistency and stable supply capacity, suitable for industrial-scale cell culture production requirements and ensuring process stability

Supply & Compliance Assurance

• Scalable Production Capacity: Our in-house production base features mature recombinant protein expression and purification capacity, reliably supporting bulk procurement and long-term supply partnerships for key accounts
• Standardized Quality System: The factory is certified to ISO 9001:2015 and follows GMP management specifications, with full manufacturing traceability. Complete quality documentation for regulatory submission is available
• Supply Chain Resilience: Fully domestic production layout is independent of international plasma supply chain fluctuations, with stable lead times to ensure production continuity
• Customized Partnership: Customized specifications, technical support and regulatory documentation support are available upon request, supporting long-term strategic cooperation

Contact Us

Anrate Biotech is dedicated to providing compliant, stable and high-quality cell culture raw material solutions for biopharmaceutical enterprises, CGT companies and media manufacturers worldwide. Please contact us for sample requests, bulk quotations or customized cooperation.

Important Notice