Recombinant Human Albumin  A Compliant Raw Material Solution for Serum-Free CGT Cell Culture

ART103S: High-Purity Recombinant Human Albumin Expressed in Pichia pastoris

ART103S recombinant human albumin from Anrate is developed with a Pichia pastoris expression system and a completely animal-free manufacturing workflow, purpose-built for serum-free and chemically defined cell culture media.

Core Quality Specifications

• Purity: ≥ 99.0%
• Molecular weight: 66.5±6.7 kDa
• Host cell protein (HCP) residue: ≤ 500 ng/g
• Endotoxin: ≤ 0.5 EU/mg
• pH range: 6.4–7.4
• Protein content: 95.0–110.0%
• Microbiological limit: ≤ 10 cfu/g
• Formulation: Lyophilized powder
• Pack sizes: 10 g / 100 g

The lyophilized powder format delivers practical advantages across the full product lifecycle:

• Long shelf life: 24 months of stability when stored at 2–8°C
• Flexible reconstitution: Dissolvable in sterile water or PBS up to 200 mg/mL, with customizable dilution and aliquoting
• Simplified logistics: Shipped with ice packs, eliminating liquid leakage and breakage risks
• Precise formulation: Direct weighing for media preparation, free from concentration variations across liquid product batches

Verified Performance Across CGT Culture Scenarios

MSC Serum-Free Culture

As the most widely studied adult stem cell type with over 1,400 registered clinical trials, MSCs have traditionally relied on FBS or human platelet lysate (hPL) in culture—materials with high batch-to-batch variation and inherent xenogeneic contamination risks. As a core component of serum-free MSC media, recombinant human albumin supports osmotic pressure homeostasis, fatty acid and metabolite transport, and antioxidant protection. The solid powder format enables accurate dosing and complete batch documentation for large-scale media manufacturing.

Immune Cell Expansion (T Cells, NK Cells, CAR-T)

For autologous CAR-T therapies, where each batch is manufactured for a single patient, raw material variability can directly impact final product quality and consistency. In serum-free immune cell expansion systems, recombinant human albumin acts as a carrier to bind and sustain the release of fatty acids and fat-soluble vitamins, maintains colloid osmotic pressure to shield cells from osmotic stress, and exerts antioxidant effects via its free cysteine (Cys-34) residue to scavenge reactive oxygen species (ROS) and reduce oxidative damage during in vitro culture.

iPSC Culture and Differentiation