FDA Recombinant HSA Human Serum Albumin For Cell Cryopreservation Animal Free

ART101S Recombinant Human Albumin: The Gold Standard for Cell Cryopreservation, Safeguarding Biopharmaceutical Development

Key Advantages at a Glance

Produced by Pichia pastoris expression system, this product is free of blood-borne viruses, mycoplasmas, prions and all animal-derived components. It prevents impurity interference, fits serum-free & animal-component-free cell culture systems, and delivers a stable, pure culture environment with minimized experimental variables.

I. ART101S: Redefine the Standard of Cell Cryoprotection

Maximum Safety: 100% Animal-component-free Protection

• Produced via Pichia pastoris expression system, free from blood-borne viruses, mycoplasma and prions (TSE/BSE) risks.
• Serum-free and animal-component-free, completely eliminating foreign protein contamination and immunogenic risks.
• Manufactured under full GMP compliance, meeting regulatory requirements of the FDA, NMPA and other global authorities, and supporting clinical trial applications.

II. Application Scenarios: Full Compatibility from R&D to Clinical Production

Cell Therapy (CAR-T, NK, MSCs and other cell products)

• Clinical-grade cell cryopreservation: Enables standardized serum-free & animal-component-free workflows to support FDA, NMPA clinical trial filings.
• Lower adverse reactions: Low-DMSO formulations reduce side effects in patients after cell infusion and improve treatment safety.
• Long-distance cold chain stability: Enhances cell stability during transportation between liquid nitrogen (-196°C) and -80°C storage.

 Vaccines & Biopharmaceuticals

• Cell bank establishment: Ideal for long-term preservation of production cell lines such as CHO and HEK293, guaranteeing stability of master and working cell banks.
• Viral vector cryopreservation: Protects activity of adenovirus, lentivirus and other viral vectors to improve transfection efficiency.
• Vaccine manufacturing: Acts as a lyoprotectant to maintain antigen conformation and immunogenicity.

Academic Research & Stem Cell Research

• Stem cell cryopreservation: Preserve pluripotency of ESCs and iPSCs and retain differentiation potential.
• Primary cell preservation: Protect fragile primary cells (neurons, hepatocytes, etc.) and raise post-thaw viability.
• 3D organoid cryopreservation: Maintain structural integrity of complex organoids for regenerative medicine research.

 III.Application Protocol: Efficient & Easy-to-use Cryopreservation Formulation

Recommended Cryopreservation Medium Formulation (Suitable for most cell types)

Standard Operating Procedures

1.Cell collection: Harvest cells in logarithmic growth phase; centrifuge at 300 g for 5 minutes to avoid cell damage from over-centrifugation.

2.Medium preparation: Mix all components at room temperature and ensure ART101S is fully dissolved.

3.Cell resuspension: Resuspend cells at a density of (1 times 10*6 sim 5 times 10*6) cells/mL; pipette gently to avoid bubble formation.

4.Freezing procedure

• For research use: 4°C (10 min) → -20°C (30 min) → Store overnight at -80°C → Long-term storage in liquid nitrogen.
• For clinical use: Use a controlled-rate freezer to cool cells at -1°C/min down to -80°C, then transfer to liquid nitrogen for long-term storage.

5.Thawing protocol: Thaw rapidly in a 37°C water bath (1–2 min). Gradually add pre-warmed complete medium, then centrifuge to remove DMSO.

4. Why Choose Anrate ART101S?